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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using Cyp4a12a Polyclonal antibody (51157-1-AP)

    WB analysis of mouse liver using 51157-1-AP (same clone as 51157-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51157-1-AP (Cyp4a12a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Cyp4a12a Polyclonal antibody (51157-1-AP)

    IHC staining of rat liver using 51157-1-AP (same clone as 51157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Cyp4a12a Polyclonal antibody (51157-1-AP)

    IHC staining of rat liver using 51157-1-AP (same clone as 51157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51157-1-PBS in a different storage buffer formulation.

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Mouse Cyp4a12a Polyclonal antibody, PBS Only

Cyp4a12a Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 51157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51157-1-PBS targets Cyp4a12a in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyp4a12a fusion protein Ag0509 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily a, polypeptide 12a
Observed molecular weight 58-70 kDa
GenBank accession numberNM_177406
Gene Symbol Cyp4a12a
Gene ID (NCBI) 277753
RRIDAB_2881255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ91WL5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Mouse Cyp4a12a oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. For modification, the MW of Cyp4a12a is about 58-70 kDa in western blot detection.