Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
51157-1-AP targets Cyp4a12a in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.
| Tested Reactivity | mouse, rat, human |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cyp4a12a fusion protein Ag0509 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 4, subfamily a, polypeptide 12a |
| Observed molecular weight | 58-70 kDa |
| GenBank accession number | NM_177406 |
| Gene Symbol | Cyp4a12a |
| Gene ID (NCBI) | 277753 |
| RRID | AB_2881255 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q91WL5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Mouse Cyp4a12a oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. For modification, the MW of Cyp4a12a is about 58-70 kDa in western blot detection.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyp4a12a antibody 51157-1-AP | Download protocol |
| IHC protocol for Cyp4a12a antibody 51157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|