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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cyclin H Monoclonal antibody

Cyclin H Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C5G11

Cat no : CL488-67065

Synonyms

CAK, CCNH, cyclin H, MO15 associated protein, p34, p37

Validation Data Gallery

Applications:
  • IF Staining of PC-3 using CL488-67065

    IF Staining of PC-3 using CL488-67065

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 Cyclin H antibody (CL488-67065, Clone: 2C5G11 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of PC-3 using CL488-67065

    FC experiment of PC-3 using CL488-67065

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Cyclin H (CL488-67065, Clone:2C5G11) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inPC-3 cells
Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 67065-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67065 targets Cyclin H in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin H fusion protein Ag28556 相同性解析による交差性が予測される生物種
Full Name cyclin H
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC005280
Gene symbol CCNH
Gene ID (NCBI) 902
RRIDAB_2919416
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cyclin H antibody CL488-67065Download protocol
FC protocol for CL Plus 488 Cyclin H antibody CL488-67065Download protocol
Standard Protocols
Click here to view our Standard Protocols