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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    WB analysis using 12738-1-AP (same clone as 12738-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12738-1-AP (Collagen Type XXV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    IF Staining of HeLa using 12738-1-AP (same clone as 12738-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    IF Staining of Neuro-2a using 12738-1-AP (same clone as 12738-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

Collagen Type XXV Polyclonal antibody, PBS Only

Collagen Type XXV Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 12738-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

COL25, COL25A1, Alzheimer disease amyloid-associated protein, CLAC-P, COL 25A1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12738-1-PBS targets Collagen Type XXV in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3634

Product name: Recombinant human COL25A1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 165-497 aa of BC036669

Sequence: GPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGTKGEQGSPGIPGMDGEQGLKGSKGDMGDPGSQWNERRKRRFWNAGSTGSFYHRPTRPTRSPWPTWPHGTSWTSWTKGSIWLRRKARIPGYRWSYGTPWPCRSQRRKR

相同性解析による交差性が予測される生物種
Full Name collagen, type XXV, alpha 1
Calculated molecular weight65 kDa
Observed molecular weight55 kDa
GenBank accession numberBC036669
Gene Symbol COL25A1
Gene ID (NCBI) 84570
RRIDAB_2291945
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXS0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Collagen alpha-1(XXV) chain is a protein that in humans is encoded by the COL25A1 gene. COL25A1 is a brain-specific membrane-bound collagen. Proteolytic processing releases CLAC, a soluble form of COL25A1 containing the extracellular collagen domains that associates with senile plaques in Alzheimer disease brains.(PMID: 11927537)

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