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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    WB analysis using 12738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12738-1-AP (Collagen Type XXV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    IF Staining of HeLa using 12738-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Collagen Type XXV Polyclonal antibody

Collagen Type XXV Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12738-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

COL25, COL25A1, COL25A 1, COL 25A1, CLAC-P

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12738-1-AP targets Collagen Type XXV in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type XXV fusion protein Ag3634 相同性解析による交差性が予測される生物種
Full Name collagen, type XXV, alpha 1
Calculated molecular weight65 kDa
Observed molecular weight55 kDa
GenBank accession numberBC036669
Gene Symbol COL25A1
Gene ID (NCBI) 84570
RRIDAB_2291945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen alpha-1(XXV) chain is a protein that in humans is encoded by the COL25A1 gene. COL25A1 is a brain-specific membrane-bound collagen. Proteolytic processing releases CLAC, a soluble form of COL25A1 containing the extracellular collagen domains that associates with senile plaques in Alzheimer disease brains.(PMID: 11927537)

Protocols

Product Specific Protocols
WB protocol for Collagen Type XXV antibody 12738-1-APDownload protocol
IF protocol for Collagen Type XXV antibody 12738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols