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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis using 85704-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis of human heart using 85704-1-RR

    human heart tissue were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis using 85704-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of human colon using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of human colon using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of mouse liver using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of mouse liver using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using COL6A1 Recombinant monoclonal antibody (85704-1-RR)

    IHC staining of mouse kidney using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using COL6A1 Recombinant monoclonal antibody (85704-1-RR)

    IHC staining of mouse colon using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COL6A1 Recombinant monoclonal antibody

COL6A1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85704-1-RR
Clone No.242936H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman kidney tissue, PC-3 cells, human heart tissue, mouse kidney tissue, mouse heart tissue
Positive IHC detected inmouse liver tissue, human colon tissue, mouse colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85704-1-RR targets COL6A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11809

Product name: Recombinant human COL6A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 770-1028 aa of BC052575

Sequence: NVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG

相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP12109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
IHC protocol for COL6A1 antibody 85704-1-RRDownload protocol
WB protocol for COL6A1 antibody 85704-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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