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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis of human heart using 85704-1-RR (same clone as 85704-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

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COL6A1 Recombinant antibody, PBS Only

COL6A1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85704-1-PBS
Clone No.242936H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85704-1-PBS targets COL6A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COL6A1 fusion protein Ag11809 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12109
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.