• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Coilin Polyclonal antibody (10967-1-AP)

    WB analysis using 10967-1-AP (same clone as 10967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Coilin Polyclonal antibody (10967-1-AP)

    WB analysis of HEK-293 using 10967-1-AP (same clone as 10967-1-PBS)

    WB result of Coilin antibody (10967-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Coilin transfected HEK-293 cells. This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Coilin Polyclonal antibody (10967-1-AP)

    WB analysis of human brain using 10967-1-AP (same clone as 10967-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Coilin Polyclonal antibody (10967-1-AP)

    IP experiment of HEK-293 using 10967-1-AP (same clone as 10967-1-PBS)

    IP result of anti-Coilin (IP:10967-1-AP, 4ug; Detection:10967-1-AP 1:10000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Coilin Polyclonal antibody (10967-1-AP)

    IHC staining of human prostate cancer using 10967-1-AP (same clone as 10967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10967-1-AP (Coilin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Coilin Polyclonal antibody (10967-1-AP)

    IHC staining of human testis using 10967-1-AP (same clone as 10967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Coilin Polyclonal antibody (10967-1-AP)

    IHC staining of human testis using 10967-1-AP (same clone as 10967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Coilin Polyclonal antibody (10967-1-AP)

    IF Staining of HeLa using 10967-1-AP (same clone as 10967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Coilin antibody (10967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Coilin Polyclonal antibody (10967-1-AP)

    FC experiment of HEK-293 using 10967-1-AP (same clone as 10967-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Coilin (10967-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

Coilin Polyclonal antibody, PBS Only

Coilin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 10967-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

COIL, CLN80, p80 coilin, p80-coilin

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10967-1-PBS targets Coilin in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1416

Product name: Recombinant human COIL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 212-576 aa of BC010385

Sequence: ANQRCSSPKGSARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELSKEEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGFLKTVGLFAGRGRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREENLFSWKGAKGRGMRGRGRGRGHPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPKKDYSLLPLLAAAPQVGEKIAFKLLELTSSYSPDVSDYKEGRILSHNPETQQVDIEILSSLPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDPRLIIESPSNTSSTEPA

相同性解析による交差性が予測される生物種
Full Name coilin
Calculated molecular weight 576 aa, 63 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
RRIDAB_2276345
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP38432
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

||
New chat

Able™

正在加载，请稍候...