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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using Chromogranin B Polyclonal antibody (14968-1-AP)

    WB analysis of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Chromogranin B Polyclonal antibody (14968-1-AP)

    WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    WB analysis of mouse brain using 14968-1-AP (same clone as 14968-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Chromogranin B Polyclonal antibody (14968-1-AP)

    WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    IHC staining of mouse pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    IHC staining of mouse pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Chromogranin B Polyclonal antibody (14968-1-AP)

    IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using 14968-1-AP (Chromogranin B antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-12 cells using Chromogranin B Polyclonal antibody (14968-1-AP)

    FC experiment of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (14968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Chromogranin B Polyclonal antibody, PBS Only

Chromogranin B Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA
Cat No. 14968-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Synonyms

CgB, CHGB, SCG1, Chromogranin-B, PE-11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14968-1-PBS targets Chromogranin B in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6833

Product name: Recombinant human CHGB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 328-677 aa of BC000375

Sequence: HSTHYRASEEEPEYGEEIKGYPGVQAPEDLEWERYRGRGSEEYRAPRPQSEESWDEEDKRNYPSLELDKMAHGYGEESEEERGLEPGKGRHHRGRGGEPRAYFMSDTREEKRFLGEGHHRVQENQMDKARRHPQGAWKELDRNYLNYGEEGAPGKWQQQGDLQDTKENREEARFQDKQYSSHHTAEKRKRLGELFNPYYDPLQWKSSHFERRDNMNDNFLEGEEENELTLNEKNFFPEYNYDWWEKKPFSEDVNWGYEKRNLARVPKLDLKRQYDRVAQLDQLLHYRKKSAEFPDFYDSEEPVSTHQEAENEKDRADQTVLTEDEKKELENLAAMDLELQKIAEKFSQRG

相同性解析による交差性が予測される生物種
Full Name chromogranin B (secretogranin 1)
Calculated molecular weight 78 kDa
Observed molecular weight 70-100 kDa
GenBank accession numberBC000375
Gene Symbol Chromogranin B
Gene ID (NCBI) 1114
RRIDAB_2081138
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP05060
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa. It has been shown that CHGB has a clear degradation band at ~50 kDa (https://www.biorxiv.org/content/10.1101/2019.12.28.890053v1.full.pdf).