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Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using Chordin Polyclonal antibody (27293-1-AP)

    WB analysis of 3T3-L1 using 27293-1-AP (same clone as 27293-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27293-1-AP (CHRD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Chordin Polyclonal antibody (27293-1-AP)

    IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Chordin Polyclonal antibody (27293-1-AP)

    IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Chordin Polyclonal antibody (27293-1-AP)

    IHC staining of rat brain using 27293-1-AP (same clone as 27293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Chordin Polyclonal antibody (27293-1-AP)

    IHC staining of rat brain using 27293-1-AP (same clone as 27293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

Chordin Polyclonal antibody, PBS Only

Chordin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27293-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27293-1-PBS targets Chordin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26001

Product name: Recombinant human CHRD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-350 aa of BC112345

Sequence: AGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQA

相同性解析による交差性が予測される生物種
Full Name chordin
Calculated molecular weight 955 aa, 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC112345
Gene Symbol Chordin
Gene ID (NCBI) 8646
RRIDAB_2880832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2X0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration.

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