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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Chordin Polyclonal antibody

Chordin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27293-1-AP

Synonyms

chordin, CHRD

Validation Data Gallery

Applications:
  • WB analysis of 3T3-L1 using 27293-1-AP

    WB analysis of 3T3-L1 using 27293-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27293-1-AP (CHRD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27293-1-AP

    IHC staining of human liver cancer using 27293-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 27293-1-AP

    IHC staining of human liver cancer using 27293-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 27293-1-AP

    IHC staining of rat brain using 27293-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 27293-1-AP

    IHC staining of rat brain using 27293-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected in3T3-L1 cells
Positive IHC detected inhuman liver cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27293-1-AP targets Chordin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chordin fusion protein Ag26001 相同性解析による交差性が予測される生物種
Full Name chordin
Calculated molecular weight 955 aa, 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC112345
Gene symbol CHRD
Gene ID (NCBI) 8646
RRIDAB_2880832
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration.

Protocols

Product Specific Protocols
WB protocol for Chordin antibody 27293-1-APDownload protocol
IHC protocol for Chordin antibody 27293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols