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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Ceruloplasmin Monoclonal antibody

Ceruloplasmin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C8A5

Cat no : CL488-66156

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-66156

    IF Staining of HeLa using CL488-66156

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Ceruloplasmin antibody (CL488-66156, Clone: 3C8A5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Ceruloplasmin/CP Ready-To-Use IHC Kit. Ceruloplasmin/CP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66156-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66156 targets Ceruloplasmin in IF applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ceruloplasmin fusion protein Ag15535 相同性解析による交差性が予測される生物種
Full Name ceruloplasmin (ferroxidase)
Calculated molecular weight 1065 aa, 122 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC146663
Gene symbol CP
Gene ID (NCBI) 1356
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Ceruloplasmin antibody CL488-66156Download protocol
Standard Protocols
Click here to view our Standard Protocols