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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Cdc20 Polyclonal ant (CL488-10252)

    IF Staining of HeLa using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated Cdc20 Polyclonal ant (CL488-10252)

    IF Staining of U2OS using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for IF/ICC
Cat No. CL488-10252

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

bA276H19.3, CDC20, CDC20A, p55CDC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10252 targets Cdc20 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0295

Product name: Recombinant human CDC20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-211 aa of BC001088

Sequence: MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSA

相同性解析による交差性が予測される生物種
Full Name cell division cycle 20 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001088
Gene Symbol CDC20
Gene ID (NCBI) 991
RRIDAB_3083867
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12834
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cdc20 antibody CL488-10252Download protocol
Standard Protocols
Click here to view our Standard Protocols