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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cardiac Troponin I Monoclonal antibody

Cardiac Troponin I Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5D6

Cat no : CL488-66376

Synonyms

Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

Validation Data Gallery

Applications:
  • IF Staining of mouse heart using CL488-66376

    IF Staining of mouse heart using CL488-66376

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Cardiac Troponin I antibody (CL488-66376, Clone: 1D5D6 ) at dilution of 1:200, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta), CoraLite®594 N-cadherin antibody (CL594-22018, red). DAPI (blue).

Tested Applications

Positive IF detected inmouse heart tissue
For other applications, we recommend the unconjugated version of this antibody, 66376-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66376 targets Cardiac Troponin I in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cardiac Troponin I fusion protein Ag16758 相同性解析による交差性が予測される生物種
Full Name troponin I type 3 (cardiac)
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC096165
Gene symbol TNNI3
Gene ID (NCBI) 7137
RRIDAB_2883322
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cardiac Troponin I antibody CL488-66376Download protocol
Standard Protocols
Click here to view our Standard Protocols