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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cadherin-16 Polyclonal antibody (15107-1-AP)

    WB analysis using 15107-1-AP (same clone as 15107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using Cadherin-16 Polyclonal antibody (15107-1-AP)

    WB analysis of mouse kidney using 15107-1-AP (same clone as 15107-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Cadherin-16 Polyclonal antibody (15107-1-AP)

    WB analysis of human kidney using 15107-1-AP (same clone as 15107-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Cadherin-16 Polyclonal antibody (15107-1-AP)

    WB analysis of HEK-293 using 15107-1-AP (same clone as 15107-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cadherin-16 Polyclonal antibody (15107-1-AP)

    IHC staining of human kidney using 15107-1-AP (same clone as 15107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Cadherin-16 Polyclonal antibody (15107-1-AP)

    IHC staining of human kidney using 15107-1-AP (same clone as 15107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Cadherin-16 Polyclonal antibody (15107-1-AP)

    IHC staining of human renal cell carcinoma using 15107-1-AP (same clone as 15107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Cadherin-16 Polyclonal antibody (15107-1-AP)

    IHC staining of human renal cell carcinoma using 15107-1-AP (same clone as 15107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15107-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cadherin-16 Polyclonal antibody, PBS Only

Cadherin-16 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15107-1-PBS targets Cadherin-16 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4343

Product name: Recombinant human CDH16 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 20-350 aa of BC027912

Sequence: AQPAELSVEVPENYGGNFPLYLTKLPLPREGAEGQIVLSGDSGKATEGPFAMDPDSGFLLVTRALDREEQAEYQLQVTLEMQDGHVLWGPQPVLVHVKDENDQVPHFSQAIYRARLSRGTRPGIPFLFLEASDRDEPGTANSDLRFHILSQAPAQPSPDMFQLEPRLGALALSPKGSTSLDHALERTYQLLVQVKDMGDQASGHQATATVEVSIIESTWVSLEPIHLAENLKVLYPHHMAQVHWSGGDVHYHLESHPPGPFEVNAEGNLYVTRELDREAQAEYLLQVRAQNSHGEDYAAPLELHVLVMDENDNVPICPPRDPTVSIPELSP

相同性解析による交差性が予測される生物種
Full Name cadherin 16, KSP-cadherin
Calculated molecular weight 90 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC027912
Gene Symbol Cadherin-16
Gene ID (NCBI) 1014
RRIDAB_10596931
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75309
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).

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