Validation Data Gallery

Filter: AllWBIHC

WB analysis using 15107-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15107-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15107-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15107-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 15107-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 15107-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human renal cell carcinoma using 15107-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 15107-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Cadherin-16 Polyclonal antibody

Cadherin-16 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15107-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, human kidney tissue, HEK-293 cells, rat kidney tissue
Positive IHC detected in	human kidney tissue, human renal cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 6 publications below

Product Information

15107-1-AP targets Cadherin-16 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4343 Product name: Recombinant human CDH16 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 20-350 aa of BC027912 Sequence: AQPAELSVEVPENYGGNFPLYLTKLPLPREGAEGQIVLSGDSGKATEGPFAMDPDSGFLLVTRALDREEQAEYQLQVTLEMQDGHVLWGPQPVLVHVKDENDQVPHFSQAIYRARLSRGTRPGIPFLFLEASDRDEPGTANSDLRFHILSQAPAQPSPDMFQLEPRLGALALSPKGSTSLDHALERTYQLLVQVKDMGDQASGHQATATVEVSIIESTWVSLEPIHLAENLKVLYPHHMAQVHWSGGDVHYHLESHPPGPFEVNAEGNLYVTRELDREAQAEYLLQVRAQNSHGEDYAAPLELHVLVMDENDNVPICPPRDPTVSIPELSP 相同性解析による交差性が予測される生物種
Full Name	cadherin 16, KSP-cadherin
Calculated molecular weight	90 kDa
Observed molecular weight	130 kDa
GenBank accession number	BC027912
Gene Symbol	Cadherin-16
Gene ID (NCBI)	1014
RRID	AB_10596931
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75309
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).

Protocols

Product Specific Protocols
IHC protocol for Cadherin-16 antibody 15107-1-AP	Download protocol
WB protocol for Cadherin-16 antibody 15107-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Int Immunopharmacol Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion Authors - Zhi Zhao View Article
mouse	WB,IF	Front Cell Dev Biol Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition. Authors - Yanhong Zhang View Article
mouse	WB,IF	J Cell Sci Pax8 controls thyroid follicular polarity through Cadherin-16. Authors - Petrina Koumarianou KD Validated View Article
human	WB	J Cancer TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. Authors - Jiaqi Dai View Article
human	WB	Oncol Lett Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion. Authors - Xiaolin Yang View Article
mouse	IF	Cell Death Dis Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis. Authors - Huzi Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15107-1-AP

WB analysis of HEK-293 using 15107-1-AP

WB analysis of mouse kidney using 15107-1-AP

WB analysis of human kidney using 15107-1-AP

IHC staining of human kidney using 15107-1-AP

IHC staining of human kidney using 15107-1-AP

IHC staining of human renal cell carcinoma using 15107-1-AP

IHC staining of human renal cell carcinoma using 15107-1-AP

Cadherin-16 Polyclonal antibody

Cadherin-16 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion

Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition.

Pax8 controls thyroid follicular polarity through Cadherin-16.

TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers.

Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis.