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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    WB analysis of A549 using 20010-1-AP (same clone as 20010-1-PBS)

    WB result of CYP4F2-Specific antibody (20010-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F2-Specific transfected A549 cells. This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    WB analysis using 20010-1-AP (same clone as 20010-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

CYP4F2-Specific Polyclonal antibody, PBS Only

CYP4F2-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20010-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20010-1-PBS targets CYP4F2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 2
Calculated molecular weight 60 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberNM_001082
Gene Symbol CYP4F2
Gene ID (NCBI) 8529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78329
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548) . It has no cross reaction to other CYP4F member.