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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    WB analysis of A549 using 20010-1-AP

    WB result of CYP4F2-Specific antibody (20010-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F2-Specific transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    WB analysis using 20010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    IHC staining of human liver using 20010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP4F2-Specific Polyclonal antibody (20010-1-AP)

    IHC staining of human liver using 20010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO Validated

CYP4F2-Specific Polyclonal antibody

CYP4F2-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20010-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CPF2, CYP4F2, CYP4F2-Specific, CYPIVF2, Cytochrome P450 4F2, Cytochrome P450 LTB omega

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HuH-7 cells, L02 cells, SMMC-7721 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20010-1-AP targets CYP4F2-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 2
Calculated molecular weight 60 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberNM_001082
Gene Symbol CYP4F2
Gene ID (NCBI) 8529
RRIDAB_3085593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548) . It has no cross reaction to other CYP4F member.

Protocols

Product Specific Protocols
WB protocol for CYP4F2-Specific antibody 20010-1-APDownload protocol
IHC protocol for CYP4F2-Specific antibody 20010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols