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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using CYP4F12 Polyclonal antibody (13243-1-AP)

    WB analysis of human placenta using 13243-1-AP (same clone as 13243-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13243-1-AP (CYP4F12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP4F12 Polyclonal antibody (13243-1-AP)

    WB analysis using 13243-1-AP (same clone as 13243-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13243-1-AP (CYP4F12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CYP4F12 Polyclonal antibody (13243-1-AP)

    WB analysis using 13243-1-AP (same clone as 13243-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13243-1-AP (CYP4F12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CYP4F12 Polyclonal antibody (13243-1-AP)

    IHC staining of human stomach cancer using 13243-1-AP (same clone as 13243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13243-1-AP (CYP4F12 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CYP4F12 Polyclonal antibody (13243-1-AP)

    IHC staining of human small intestine using 13243-1-AP (same clone as 13243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13243-1-AP (CYP4F12 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 13243-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP4F12 Polyclonal antibody (13243-1-AP)

    IF Staining of HepG2 using 13243-1-AP (same clone as 13243-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP4F12 antibody (13243-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13243-1-PBS in a different storage buffer formulation.

CYP4F12 Polyclonal antibody, PBS Only

CYP4F12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13243-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CYPIVF12, Cytochrome P450 4F12, EC:1.14.14.1, UNQ568/PRO1129

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13243-1-PBS targets CYP4F12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3957

Product name: Recombinant human CYP4F12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-524 aa of BC035350

Sequence: ILELSALVEKRSQHILQHMDFLYYLSHDGRRFHRACRLVHDFTDAVIRERRRTLPTQGIDDFFKDKAKSKTLDFIDVLLLSKDEDGKALSDEDIRAEADTFMFGGHDTTASGLSWVLYNLARHPEYQERCRQEVQELLKDRDPKEIEWDDLAQLPFLTMCVKESLRLHPPAPFISRCCTQDIVLPDGRVIPKGITCLIDIIGVHHNPTVWPDPEVYDPFRFDPENSKGRSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALMLLHFRFLPDHTEPRRKLELIMRAEGGLWLRVEPLNVSLQ

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 12
Calculated molecular weight 524 aa, 60 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC035350
Gene Symbol CYP4F12
Gene ID (NCBI) 66002
RRIDAB_2088412
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCS2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP4F12(Cytochrome P450 4F12) Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. It expresses a band of 60KDa in adult and fetal liver, kidney, placenta at term, seminal vesicles, and the prostate gland(PMID:15078342). And it can be phosphorylated in vivo(PMID:18828626).

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