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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP4F12 Polyclonal antibody (13243-1-AP)

    WB analysis using 13243-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13243-1-AP (CYP4F12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CYP4F12 Polyclonal antibody (13243-1-AP)

    WB analysis of human placenta using 13243-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13243-1-AP (CYP4F12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CYP4F12 Polyclonal antibody (13243-1-AP)

    IHC staining of human stomach cancer using 13243-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13243-1-AP (CYP4F12 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CYP4F12 Polyclonal antibody (13243-1-AP)

    IHC staining of human small intestine using 13243-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13243-1-AP (CYP4F12 antibody) at dilution of 1:500 (under 40x lens).

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CYP4F12 Polyclonal antibody

CYP4F12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13243-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CYPIVF12, Cytochrome P450 4F12, EC:1.14.14.1, UNQ568/PRO1129

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, human placenta tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13243-1-AP targets CYP4F12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP4F12 fusion protein Ag3957 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily F, polypeptide 12
Calculated molecular weight 524 aa, 60 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC035350
Gene Symbol CYP4F12
Gene ID (NCBI) 66002
RRIDAB_2088412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP4F12(Cytochrome P450 4F12) Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. It expresses a band of 60KDa in adult and fetal liver, kidney, placenta at term, seminal vesicles, and the prostate gland(PMID:15078342). And it can be phosphorylated in vivo(PMID:18828626).

Protocols

Product Specific Protocols
WB protocol for CYP4F12 antibody 13243-1-APDownload protocol
IHC protocol for CYP4F12 antibody 13243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

CYP4F12 is a potential biomarker and inhibits cell migration of head and neck squamous cell carcinoma via EMT pathway

Authors - Wenming Jia
View Article