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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CYP4A11 Polyclonal antibody (11688-1-AP)

    WB analysis of HEK-293 using 11688-1-AP (same clone as 11688-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    WB analysis of rat kidney using 11688-1-AP (same clone as 11688-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    WB analysis of mouse kidney using 11688-1-AP (same clone as 11688-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    WB analysis of mouse kidney using 11688-1-AP (same clone as 11688-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    IHC staining of human kidney using 11688-1-AP (same clone as 11688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    IHC staining of human kidney using 11688-1-AP (same clone as 11688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    IHC staining of human kidney using 11688-1-AP (same clone as 11688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    IHC staining of human kidney using 11688-1-AP (same clone as 11688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    IHC staining of human liver using 11688-1-AP (same clone as 11688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP4A11 Polyclonal antibody (11688-1-AP)

    IHC staining of human liver using 11688-1-AP (same clone as 11688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11688-1-PBS in a different storage buffer formulation.

CYP4A11 Polyclonal antibody, PBS Only

CYP4A11 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11688-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, CP4Y, CYP4A14, CYP4A2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11688-1-PBS targets CYP4A11 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2300

Product name: Recombinant human CYP4A11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-215 aa of BC022851

Sequence: MSVSVLSPSRLLGDVSGILQAASLLILLLLLIKAVQLYLHRQWLLKALQQFPCPPSHWLFGHIQELQQDQELQRIQKWVETFPSACPHWLWGGKVRVQLYDPDYMKVILGRSDPKSHGSYRFLAPWIGYGLLLLNGQTWFQHRRMLTPAFHYDILKPYVGLMADSVRVMLDKWEELLGQDSPLEVFQHVSLMTLDTIMKCAFRHWQRAQHSRHLP

相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily A, polypeptide 11
Calculated molecular weight 519 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC022851
Gene Symbol CYP4A11
Gene ID (NCBI) 1579
RRIDAB_10804774
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02928
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CYP4A is a member of the CYP4 enzyme family and is involved in the metabolism of medium- and long-chain fatty acids, such as arachidonic acid, palmitate, laurate and compounds with highly regionally selective hydroxylated terminal ω-carbon (PMID: 2554928). CYP4A11 is involved in the development of nonalcoholic fatty liver disease via ROS-induced lipid peroxidation and inflammation (PMID: 32124935). CYP4A11 primarily metabolizes endobiotics and catalyzes the ω-hydroxylation of fatty acids in human liver and kidney tissues (PMID: 34594219). The human CYP4A11 is homologous to mouse CYP4A14 (PMID: 38356602).

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