Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, rat kidney tissue, mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
11688-1-AP targets CYP4A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2300 Product name: Recombinant human CYP4A11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC022851 Sequence: MSVSVLSPSRLLGDVSGILQAASLLILLLLLIKAVQLYLHRQWLLKALQQFPCPPSHWLFGHIQELQQDQELQRIQKWVETFPSACPHWLWGGKVRVQLYDPDYMKVILGRSDPKSHGSYRFLAPWIGYGLLLLNGQTWFQHRRMLTPAFHYDILKPYVGLMADSVRVMLDKWEELLGQDSPLEVFQHVSLMTLDTIMKCAFRHWQRAQHSRHLP 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 4, subfamily A, polypeptide 11 |
| Calculated molecular weight | 519 aa, 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC022851 |
| Gene Symbol | CYP4A11 |
| Gene ID (NCBI) | 1579 |
| RRID | AB_10804774 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02928 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP4A is a member of the CYP4 enzyme family and is involved in the metabolism of medium- and long-chain fatty acids, such as arachidonic acid, palmitate, laurate and compounds with highly regionally selective hydroxylated terminal ω-carbon (PMID: 2554928). CYP4A11 is involved in the development of nonalcoholic fatty liver disease via ROS-induced lipid peroxidation and inflammation (PMID: 32124935). CYP4A11 primarily metabolizes endobiotics and catalyzes the ω-hydroxylation of fatty acids in human liver and kidney tissues (PMID: 34594219). The human CYP4A11 is homologous to mouse CYP4A14 (PMID: 38356602).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP4A11 antibody 11688-1-AP | Download protocol |
| WB protocol for CYP4A11 antibody 11688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. | ||
Food Funct Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats. | ||
Food Funct Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats. | ||
Forensic Sci Int Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia. | ||
Chem Biol Interact Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy. | ||
Transl Res Aldehyde dehydrogenase 2 attenuates renal injury through inhibiting CYP4A expression |
