Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, mouse lung tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88462-1-RR targets CYP1A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3990 Product name: Recombinant human CYP1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 115-465 aa of BC023019 Sequence: ISNGQSMSFSPDSGPVWAARRRLAQNGLKSFSIASDPASSTSCYLEEHVSKEAEVLISTLQELMAGPGHFNPYRYVVVSVTNVICAICFGRRYDHNHQELLSLVNLNNNFGEVVGSGNPADFIPILRYLPNPSLNAFKDLNEKFYSFMQKMVKEHYKTFEKGHIRDITDSLIEHCQEKQLDENANVQLSDEKIINIVLDLFGAGFDTVTTAISWSLMYLVMNPRVQRKIQEELDTVIGRSRRPRLSDRSHLPYMEAFILETFRHSSFVPFTIPHSTTRDTSLKGFYIPKGRCVFVNQWQINHDQKLWVNPSEFLPERFLTPDGAIDKVLSEKVIIFGMGKRKCIGETIARW 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 1, subfamily A, polypeptide 1 |
| Calculated molecular weight | 512 aa, 58 kDa |
| Observed molecular weight | 50-58 kDa |
| GenBank accession number | BC023019 |
| Gene Symbol | CYP1A1 |
| Gene ID (NCBI) | 1543 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04798 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP1A1 is a phase I xenobiotic-metabolizing enzyme that bioactivates environmental procarcinogens, particularly polycyclic aromatic hydrocarbons (PAHs), into reactive intermediates that can initiate carcinogenesis. Its expression is primarily regulated at the transcriptional level by the aryl hydrocarbon receptor (AHR), which upon ligand binding induces CYP1A1 via xenobiotic response elements. Genetic polymorphisms in the human CYP1A1 gene have been linked to increased susceptibility to lung and other cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP1A1 antibody 88462-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |