Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85690-5-RR targets CYP17A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CYP17A1 fusion protein Ag5899 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome P450, family 17, subfamily A, polypeptide 1 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 50-57 kDa |
| GenBank accession number | BC062997 |
| Gene Symbol | CYP17A1 |
| Gene ID (NCBI) | 1586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05093 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The human cytochrome P450c17 enzyme is encoded by the CYP17A1 gene. It is the qualitative regulator of steroidogenesis in the human adrenals.In its absence, aldosterone is produced in the human adrenal zona glomerulosa .The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID;19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme(PMID:15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer (PMID 27505042, PMID 27505042).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP17A1 antibody 85690-5-RR | Download protocol |
| IHC protocol for CYP17A1 antibody 85690-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |