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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP17A1 Recombinant antibody (85690-5-RR)

    WB analysis using 85690-5-RR (same clone as 85690-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85690-5-RR (CYP17A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CYP17A1 Recombinant antibody (85690-5-RR)

    IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CYP17A1 Recombinant antibody (85690-5-RR)

    IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85690-5-RR

    Affinity and Kinetic Characterization of 85690-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85690-5-RR against Human CYP17A1 were performed. The affinity constant is below 1 pM.

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CYP17A1 Recombinant antibody, PBS Only

CYP17A1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85690-5-PBS
Clone No.243076D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85690-5-PBS targets CYP17A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CYP17A1 fusion protein Ag5899 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 17, subfamily A, polypeptide 1
Calculated molecular weight 57 kDa
Observed molecular weight 50-57 kDa
GenBank accession numberBC062997
Gene Symbol CYP17A1
Gene ID (NCBI) 1586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05093
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cytochrome P450 17A1 (CYP17A1) is a critical steroidogenic enzyme, essential for producing glucocorticoids and sex hormones (PMID: 33781841). The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID: 19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme (PMID: 15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer (PMID: 27505042).