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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse serum using CXCL4/PF4 Recombinant antibody (85647-1-RR)

    WB analysis of mouse serum using 85647-1-RR

    mouse serum was subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using CXCL4/PF4 Recombinant antibody (85647-1-RR)

    WB analysis of human peripheral blood platelets using 85647-1-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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CXCL4/PF4 Recombinant antibody

CXCL4/PF4 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85647-1-RR
Clone No.243087F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse serum, human peripheral blood platelets

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85647-1-RR targets CXCL4/PF4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name platelet factor 4
Calculated molecular weight11 kDa
Observed molecular weight11 kDa
GenBank accession numberNM_002619.4
Gene Symbol PF4
Gene ID (NCBI) 5196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation

Protocols

Product Specific Protocols
WB protocol for CXCL4/PF4 antibody 85647-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols