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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse serum using CXCL4/PF4 Recombinant antibody (85647-1-RR)

    WB analysis of mouse serum using 85647-1-RR (same clone as 85647-1-PBS)

    mouse serum was subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using CXCL4/PF4 Recombinant antibody (85647-1-RR)

    WB analysis of human peripheral blood platelets using 85647-1-RR (same clone as 85647-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85647-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85647-1-RR

    Affinity and Kinetic Characterization of 85647-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84647-1-RR against Human CXCL4/PF4 were performed. The affinity constant is 0.625 nM.

CXCL4/PF4 Recombinant monoclonal antibody, PBS Only

CXCL4/PF4 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85647-1-PBS
Clone No.243087F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85647-1-PBS targets CXCL4/PF4 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2129

Product name: Recombinant Human CXCL4/PF4 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 32-101 aa of NM_002619.4

Sequence: EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

相同性解析による交差性が予測される生物種
Full Name platelet factor 4
Calculated molecular weight11 kDa
Observed molecular weight11 kDa
GenBank accession numberNM_002619.4
Gene Symbol PF4
Gene ID (NCBI) 5196
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02776
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation

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