Validation Data Gallery
Tested Applications
| Positive WB detected in | TT cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87928-3-RR targets CXCL13/BCA1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg7258 Product name: Recombinant human CXCL13 protein Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 23-109 aa of NM_006419.2 Sequence: VLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNGCPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKRSSSTLPVPVFKRKIP 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-X-C motif) ligand 13 |
| Calculated molecular weight | 13 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | NM_006419.2 |
| Gene Symbol | CXCL13/BCA1 |
| Gene ID (NCBI) | 10563 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q53X90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CXCL13 (C-X-C motif chemokine ligand 13), also known as B cell-attracting chemokine 1 (BCA-1) or B-lymphocyte chemoattractant (BLC), is a small cytokine belonging to the CXC chemokine family. It was independently discovered by two research groups in 1998-Legler et al. identified it as BCA-1, while Gunn et al. described it as BLC. CXCL13 is primarily secreted by follicular dendritic cells (FDCs) within B-cell follicles of secondary lymphoid organs, including lymph nodes, spleen, and Peyer's patches.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CXCL13/BCA1 antibody 87928-3-RR | Download protocol |
| WB protocol for CXCL13/BCA1 antibody 87928-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |