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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXADR Recombinant antibody (86186-1-RR)

    WB analysis using 86186-1-RR (same clone as 86186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CXADR Recombinant antibody (86186-1-RR)

    WB analysis using 86186-1-RR (same clone as 86186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CXADR Recombinant antibody (86186-1-RR)

    IHC staining of mouse heart using 86186-1-RR (same clone as 86186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using CXADR Recombinant antibody (86186-1-RR)

    IHC staining of rat heart using 86186-1-RR (same clone as 86186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86186-1-RR

    Affinity and Kinetic Characterization of 86186-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86186-1-RR against Human CXADR were performed. The affinity constant is 96.1 pM.

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CXADR Recombinant antibody, PBS Only

CXADR Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86186-1-PBS
Clone No.250811C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CAR, Coxsackievirus B-adenovirus receptor, CVB3 binding protein, CVB3-binding protein, HCAR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86186-1-PBS targets CXADR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXADR fusion protein Ag34346 相同性解析による交差性が予測される生物種
Full Name coxsackie virus and adenovirus receptor
Calculated molecular weight 365 aa, 40 kDa
Observed molecular weight45-55 kDa
GenBank accession numberBC003684
Gene Symbol CXADR
Gene ID (NCBI) 1525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78310
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Coxsackievirus and adenovirus receptor (CXADR) is a member of junctional adhesion molecules, which are glycosylated transmembrane proteins that belong to the Ig superfamily (PMID: 17916321). CXADR plays essential roles in blastocyst formation, including cavitation and the proper subcellular localization of tight junction proteins into the apical edge of the outer blastomeres (PMID: 26729920). It is also involved in the transepithelial migration of leukocytes through adhesive interactions with junctional adhesion molecule-like protein (JAML) (PMID: 15800062).