Validation Data Gallery
Tested Applications
| Positive WB detected in | HaCaT cells, mouse heart tissue, rat heart tissue, mouse small intestine tissue, BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86186-1-RR targets CXADR in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34346 Product name: Recombinant human CXADR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-365 aa of BC003684 Sequence: MALLLCFVLLCGVVDFARSLSITTPEEMIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPADNQKVDQVIILYSGDKIYDDYYPDLKGRVHFTSNDLKSGDASINVTNLQLSDIGTYQCKVKKAPGVANKKIHLVVLVKPSGARCYVDGSEEIGSDFKIKCEPKEGSLPLQYEWQKLSDSQKMPTSWLAEMTSSVISVKNASSEYSGTYSCTVRNRVGSDQCLLRLNVVPPSNKAGLIAGAIIGTLLALALIGLIIFCCRKKRREEKYEKEVHHDIREDVPPPKSRTSTARSYIGSNHSSLGSMSPSNMEGYSKTQYNQVPSEDFERTPQSPTLPPAKVAAPNLSRMGAIPVMIPAQSKDGSIV* 相同性解析による交差性が予測される生物種 |
| Full Name | coxsackie virus and adenovirus receptor |
| Calculated molecular weight | 365 aa, 40 kDa |
| Observed molecular weight | 45-55 kDa |
| GenBank accession number | BC003684 |
| Gene Symbol | CXADR |
| Gene ID (NCBI) | 1525 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P78310 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Coxsackievirus and adenovirus receptor (CXADR) is a member of junctional adhesion molecules, which are glycosylated transmembrane proteins that belong to the Ig superfamily (PMID: 17916321). CXADR plays essential roles in blastocyst formation, including cavitation and the proper subcellular localization of tight junction proteins into the apical edge of the outer blastomeres (PMID: 26729920). It is also involved in the transepithelial migration of leukocytes through adhesive interactions with junctional adhesion molecule-like protein (JAML) (PMID: 15800062).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CXADR antibody 86186-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |