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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL9 Polyclonal antibody (31220-1-AP)

    WB analysis using 31220-1-AP (same clone as 31220-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31220-1-AP (CUL9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31220-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CUL9 Polyclonal antibody (31220-1-AP)

    IF Staining of U2OS using 31220-1-AP (same clone as 31220-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CUL9 antibody (31220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31220-1-PBS in a different storage buffer formulation.

CUL9 Polyclonal antibody, PBS Only

CUL9 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31220-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31220-1-PBS targets CUL9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35405

Product name: Recombinant human CUL9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2284-2517 aa of BC028159

Sequence: SKAARQEKRFQDYNERCTFHHQAREFAVNLRNRVSAIHEVPPPRSFTFLNDACQGLEQARKVLAYACVYSFYSQDAEYMDVVEQQTENLELHTNALQILLEETLLRCRDLASSLRLLRADCLSTGMELLRRIQERLLAILQHSAQDFRVGLQSPSVEAWEAKGPNMPGSQPQASSGPEAEEEEEDDEDDVPEWQQDEFDEELDNDSFSYDESENLDQETFFFGDEEEDEDEAYD

相同性解析による交差性が予測される生物種
Full Name cullin 9
Observed molecular weight268 kDa
GenBank accession numberBC028159
Gene Symbol CUL9
Gene ID (NCBI) 23113
RRIDAB_3669906
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8IWT3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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