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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL9 Polyclonal antibody (31220-1-AP)

    WB analysis using 31220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31220-1-AP (CUL9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CUL9 Polyclonal antibody (31220-1-AP)

    IF Staining of U2OS using 31220-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CUL9 antibody (31220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CUL9 Polyclonal antibody

CUL9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31220-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

PARC, KIAA0708, H7AP1, cullin 9, CUL 9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, A431 cells, Jurkat cells, mouse testis tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31220-1-AP targets CUL9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL9 fusion protein Ag35405 相同性解析による交差性が予測される生物種
Full Name cullin 9
Observed molecular weight268 kDa
GenBank accession numberBC028159
Gene Symbol CUL9
Gene ID (NCBI) 23113
RRIDAB_3669906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8IWT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CUL9 antibody 31220-1-APDownload protocol
IF protocol for CUL9 antibody 31220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols