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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CUL4B Polyclonal antibody (CL594-12916)

    IF Staining of HeLa using CL594-12916

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 CUL4B antibody (CL594-12916) at dilution of 1:200, CL488-phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CUL4B Polyclonal antibody (CL594-12916)

    IF Staining of HepG2 using CL594-12916

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 CUL4B antibody (CL594-12916) at dilution of 1:200, CL488-phalloidin (green).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated CUL4B Polyclonal antibody (CL594-12916)

    FC experiment of HeLa using CL594-12916

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CUL4B (CL594-12916) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-12916

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

cullin 4B, Cullin-4B, KIAA0695

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-12916 targets CUL4B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3563

Product name: Recombinant human CUL4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC036216

Sequence: MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGIL

相同性解析による交差性が予測される生物種
Full Name cullin 4B
Calculated molecular weight 913 aa, 104 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_2919790
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13620
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

Protocols

Product Specific Protocols
IF protocol for CL594 CUL4B antibody CL594-12916Download protocol
FC protocol for CL594 CUL4B antibody CL594-12916Download protocol
Standard Protocols
Click here to view our Standard Protocols