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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    WB analysis of mouse pancreas using 14856-1-AP (same clone as 14856-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using CTRL Polyclonal antibody (14856-1-AP)

    WB analysis of BxPC-3 using 14856-1-AP (same clone as 14856-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    WB analysis of mouse pancreas using 14856-1-AP (same clone as 14856-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    IHC staining of human pancreas using 14856-1-AP (same clone as 14856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRL Polyclonal antibody (14856-1-AP)

    IHC staining of human pancreas using 14856-1-AP (same clone as 14856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CTRL Polyclonal antibody (14856-1-AP)

    IF Staining of BxPC-3 using 14856-1-AP (same clone as 14856-1-PBS)

    Immunofluorescent analysis of BxPC-3 cells using 14856-1-AP (CTRL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

CTRL Polyclonal antibody, PBS Only

CTRL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14856-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14856-1-PBS targets CTRL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6644

Product name: Recombinant human CTRL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-264 aa of BC063475

Sequence: MLLLSLTLSLVLLGSSWGCGIPAIKPALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHCNVSPGRHFVVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTTMNNDVTLLKLASPAQYTTRISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQCRQYWGSSITDSMICAGGAGASSCQGDSGGPLVCQKGNTWVLIGIVSWGTKNCNVRAPAVYTRVSKFSTWINQVIAYN

相同性解析による交差性が予測される生物種
Full Name chymotrypsin-like
Calculated molecular weight 28 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC063475
Gene Symbol CTRL
Gene ID (NCBI) 1506
RRIDAB_10640303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP40313
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.

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