Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse pancreas tissue, BxPC-3 cells |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
14856-1-AP targets CTRL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6644 Product name: Recombinant human CTRL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC063475 Sequence: MLLLSLTLSLVLLGSSWGCGIPAIKPALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHCNVSPGRHFVVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTTMNNDVTLLKLASPAQYTTRISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQCRQYWGSSITDSMICAGGAGASSCQGDSGGPLVCQKGNTWVLIGIVSWGTKNCNVRAPAVYTRVSKFSTWINQVIAYN 相同性解析による交差性が予測される生物種 |
| Full Name | chymotrypsin-like |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 25-28 kDa |
| GenBank accession number | BC063475 |
| Gene Symbol | CTRL |
| Gene ID (NCBI) | 1506 |
| RRID | AB_10640303 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CTRL antibody 14856-1-AP | Download protocol |
| IHC protocol for CTRL antibody 14856-1-AP | Download protocol |
| WB protocol for CTRL antibody 14856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Thromb Haemost Plasma Proteolytic Cascade Activation during Neonatal Cardiopulmonary Bypass Surgery. | ||
Biol Open Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems. | ||
Oncotarget The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression. |
