• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    WB analysis using 87022-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IHC staining of rat heart using 87022-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of rat heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of rat heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of mouse heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of mouse heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 87022-1-RR

    Affinity and Kinetic Characterization of 87022-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87022-1-RR against Human CTNNA3 were performed. The affinity constant is 3.11 nM.

CTNNA3 Recombinant monoclonal antibody

CTNNA3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 87022-1-RR
Clone No.252141F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF-P, ELISA

Synonyms

Alpha T catenin, Alpha T-catenin, Cadherin-associated protein, Catenin alpha 3, Catenin alpha-3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MDCK cells, C2C12 cells, C6 cells, PC-12 cells, mouse testis tissue, rat brain tissue
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat heart tissue, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87022-1-RR targets CTNNA3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5008

Product name: Recombinant human CTNNA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-376 aa of BC030646

Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL

相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), alpha 3
Calculated molecular weight 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC030646
Gene Symbol CTNNA3
Gene ID (NCBI) 29119
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UI47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403).

Protocols

Product Specific Protocols
IF protocol for CTNNA3 antibody 87022-1-RRDownload protocol
IHC protocol for CTNNA3 antibody 87022-1-RRDownload protocol
WB protocol for CTNNA3 antibody 87022-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...