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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    WB analysis using 87022-1-RR (same clone as 87022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IHC staining of rat heart using 87022-1-RR (same clone as 87022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of rat heart using 87022-1-RR (same clone as 87022-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of rat heart using 87022-1-RR (same clone as 87022-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of mouse heart using 87022-1-RR (same clone as 87022-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CTNNA3 Recombinant monoclonal antibody (87022-1-RR)

    IF Staining of mouse heart using 87022-1-RR (same clone as 87022-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 87022-1-RR

    Affinity and Kinetic Characterization of 87022-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87022-1-RR against Human CTNNA3 were performed. The affinity constant is 3.11 nM.

CTNNA3 Recombinant monoclonal antibody, PBS Only

CTNNA3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 87022-1-PBS
Clone No.252141F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Alpha T catenin, Alpha T-catenin, Cadherin-associated protein, Catenin alpha 3, Catenin alpha-3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87022-1-PBS targets CTNNA3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5008

Product name: Recombinant human CTNNA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-376 aa of BC030646

Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL

相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), alpha 3
Calculated molecular weight 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC030646
Gene Symbol CTNNA3
Gene ID (NCBI) 29119
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UI47
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403).

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