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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTAM Recombinant antibody (84068-4-RR)

    WB analysis using 84068-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84068-4-RR (CRTAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRTAM Recombinant antibody (84068-4-RR)

    IHC staining of mouse brain using 84068-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84068-4-RR (CRTAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CRTAM Recombinant antibody (84068-4-RR)

    IF Staining of mouse brain using 84068-4-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CRTAM antibody (84068-4-RR, Clone: 241280C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84068-4-RR

    Affinity and Kinetic Characterization of 84068-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84068-4-RR against Human CRTAM were performed. The affinity constant is 0.427 nM.

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CRTAM/CD355 Recombinant antibody

CRTAM/CD355 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84068-4-RR
Clone No.241280C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355, 241280C4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84068-4-RR targets CRTAM/CD355 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cytotoxic and regulatory T cell molecule
Calculated molecular weight45 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_019604.4
Gene Symbol CRTAM
Gene ID (NCBI) 56253
RRIDAB_3671636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95727-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223). The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa (PMID: 16300832).

Protocols

Product Specific Protocols
WB protocol for CRTAM/CD355 antibody 84068-4-RRDownload protocol
IHC protocol for CRTAM/CD355 antibody 84068-4-RRDownload protocol
IF protocol for CRTAM/CD355 antibody 84068-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols