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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTAM/CD355 Recombinant antibody (84068-4-RR)

    WB analysis using 84068-4-RR (same clone as 84068-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84068-4-RR (CRTAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84068-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRTAM/CD355 Recombinant antibody (84068-4-RR)

    IHC staining of mouse brain using 84068-4-RR (same clone as 84068-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84068-4-RR (CRTAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84068-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CRTAM/CD355 Recombinant antibody (84068-4-RR)

    IF Staining of mouse brain using 84068-4-RR (same clone as 84068-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CRTAM antibody (84068-4-RR, Clone: 241280C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84068-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84068-4-RR

    Affinity and Kinetic Characterization of 84068-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84068-4-RR against Human CRTAM were performed. The affinity constant is 0.427 nM.

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CRTAM/CD355 Recombinant antibody, PBS Only

CRTAM/CD355 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84068-4-PBS
Clone No.241280C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355, 241280C4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84068-4-PBS targets CRTAM/CD355 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cytotoxic and regulatory T cell molecule
Calculated molecular weight45 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_019604.4
Gene Symbol CRTAM
Gene ID (NCBI) 56253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95727-1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223). The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa (PMID: 16300832).