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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated CRMP5 Monoclonal ant (CL488-68272)

    IF Staining of SH-SY5Y using CL488-68272

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP5 antibody (CL488-68272, Clone: 3F2D8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CRMP5 Monoclonal antibody

CRMP5 Monoclonal Antibody for IF/ICC
Cat No. CL488-68272
Clone No.3F2D8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Synonyms

DPYSL5, 3F2D8, CRMP 5, CRMP3-associated molecule, CRMP-5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68272 targets CRMP5 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0783

Product name: Recombinant human DPYSL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 265-564 aa of BC002874

Sequence: KVVLAETTTAHATLTGLHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSGVQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQGGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRSSGIW

相同性解析による交差性が予測される生物種
Full Name dihydropyrimidinase-like 5
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC002874
Gene Symbol CRMP5
Gene ID (NCBI) 56896
RRIDAB_3672986
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BPU6
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collapsin response mediator proteins (CRMPs) are a family consisting of five homologous cytosolic phosphoproteins (CRMP1-5). All five CRMPs are abundantly expressed in the developing brain and the adult nervous system. CRMP5 is phylogenetically divergent from the other four CRMPs, sharing only 50% homology; this protein plays an important role in modulating filopodial dynamics and, in turn, restricting neuronal growth cone development.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CRMP5 antibody CL488-68272Download protocol
Standard Protocols
Click here to view our Standard Protocols