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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CRM1 Monoclonal antibody

CRM1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D3B6

Cat no : CL488-66763

Synonyms

CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1

Validation Data Gallery

Applications:
  • WB analysis using CL488-66763

    WB analysis using CL488-66763

    HeLa cells were subjected to SDS PAGE followed by western blot with CL488-66763 (CRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CRM1 Ready-To-Use IHC Kit. CRM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66763-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

CL488-66763 targets CRM1 in WB applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRM1 fusion protein Ag27337 相同性解析による交差性が予測される生物種
Full Name exportin 1 (CRM1 homolog, yeast)
Calculated molecular weight 1071 aa, 123 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC032847
Gene symbol XPO1
Gene ID (NCBI) 7514
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 CRM1 antibody CL488-66763Download protocol
Standard Protocols
Click here to view our Standard Protocols