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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CRABP2 Monoclonal antibody

CRABP2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A5F3

Cat no : CL594-66468

Synonyms

CRABP II, CRABP2, RBP6

Validation Data Gallery

Applications:
  • IF Staining of human skin cancer using CL594-66468

    IF Staining of human skin cancer using CL594-66468

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CoraLite®594-conjugated CRABP2 antibody (CL594-66468, Clone: 1A5F3 ) at dilution of 1:100.

  • FC experiment of MCF-7 using CL594-66468

    FC experiment of MCF-7 using CL594-66468

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CRABP2 (CL594-66468, Clone:1A5F3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman skin cancer tissue
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66468-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66468 targets CRABP2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRABP2 fusion protein Ag0309 相同性解析による交差性が予測される生物種
Full Name cellular retinoic acid binding protein 2
Calculated molecular weight 16 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC001109
Gene symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2919998
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
IF protocol for CL594 CRABP2 antibody CL594-66468Download protocol
FC protocol for CL594 CRABP2 antibody CL594-66468Download protocol
Standard Protocols
Click here to view our Standard Protocols