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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using CoraLite®594-conjugated CRABP2 Monoclonal antibody (CL594-66468)

    IF Staining of human skin cancer using CL594-66468

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CoraLite®594-conjugated CRABP2 antibody (CL594-66468, Clone: 1A5F3 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated CRABP2 Monoclonal antibody (CL594-66468)

    FC experiment of MCF-7 using CL594-66468

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CRABP2 (CL594-66468, Clone:1A5F3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CRABP2 Monoclonal antibody

CRABP2 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-66468
Clone No.1A5F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman skin cancer tissue
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66468 targets CRABP2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0309

Product name: Recombinant human CRABP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-138 aa of BC001109

Sequence: MPNFSGNWKIIRSENFEELLKVLGVNVMLRKIAVAAASKPAVEIKQEGDTFYIKTSTTVRTTEINFKVGEEFEEQTVDGRPCKSLVKWESENKMVCEQKLLKGEGPKTSWTRELTNDGELILTMTADDVVCTRVYVRE

相同性解析による交差性が予測される生物種
Full Name cellular retinoic acid binding protein 2
Calculated molecular weight 16 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2919998
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP29373
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
IF protocol for CL594 CRABP2 antibody CL594-66468Download protocol
Standard Protocols
Click here to view our Standard Protocols