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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD35 Monoclonal antibody

CD35 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B8E6

Cat no : CL488-68033

Synonyms

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

Validation Data Gallery

Applications:
  • IF Staining of human appendicitis using CL488-68033

    IF Staining of human appendicitis using CL488-68033

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 CD35 antibody (CL488-68033, Clone: 1B8E6 ) at dilution of 1:200.

  • IF Staining of human appendicitis using CL488-68033

    IF Staining of human appendicitis using CL488-68033

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 CD35 antibody (CL488-68033, Clone: 1B8E6 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68033-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-68033 targets CD35 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD35 fusion protein Ag30268 相同性解析による交差性が予測される生物種
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Calculated molecular weight 224 kDa
GenBank accession numberNM_000573
Gene symbol CR1
Gene ID (NCBI) 1378
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. A soluble form of CD35 has been found in plasma (PMID: 3156931). It has been reported that soluble CD35 is produced by a proteolytic cleavage in the C-terminal region of CD35 transmembrane domain (PMID: 9405292).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD35 antibody CL488-68033Download protocol
Standard Protocols
Click here to view our Standard Protocols