Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-82701-3 targets CPT1B in IF-P applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag17840 Product name: Recombinant human CPT1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 110-250 aa of BC131570 Sequence: FNTTRIPGKDTDVLQHLSDSRHVAVYHKGRFFKLWLYEGARLLKPQDLEMQFQRILDDPSPPQPGEEKLAALTAGGRVEWAQARQAFFSSGKNKAALEAIERAAFFVALDEESYSYDPEDEASLSLYGKALLHGNCYNRWF 相同性解析による交差性が予測される生物種 |
| Full Name | carnitine palmitoyltransferase 1B (muscle) |
| Calculated molecular weight | 567 aa, 64 kDa |
| Observed molecular weight | 70-85 kDa |
| GenBank accession number | BC131570 |
| Gene Symbol | CPT1B |
| Gene ID (NCBI) | 1375 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92523 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CPT1B (carnitine palmitoyltransferase 1B) is an isoform of the mitochondrial enzyme carnitine palmitoyltransferase 1, primarily expressed in skeletal muscle and heart tissues, where it plays a critical role in the regulation of long-chain fatty acid oxidation. By catalyzing the conversion of long-chain acyl-CoA to acyl-carnitine, which is then transported into the mitochondrial matrix for β-oxidation, CPT1B serves as a rate-limiting enzyme in energy metabolism, particularly under conditions of high energy demand such as exercise. Its activity is allosterically inhibited by malonyl-CoA, allowing tight metabolic control, and genetic variations or dysregulation of CPT1B have been implicated in metabolic disorders, insulin resistance, and obesity, highlighting its importance in maintaining systemic energy homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 CPT1B antibody CL647-82701-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |