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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis using 55195-1-AP (same clone as 55195-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of HeLa using 55195-1-AP (same clone as 55195-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of HEK-293 using 55195-1-AP (same clone as 55195-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of mouse brain using 55195-1-AP (same clone as 55195-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    WB analysis of mouse brain using 55195-1-AP (same clone as 55195-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human gliomas using 55195-1-AP (same clone as 55195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human gliomas using 55195-1-AP (same clone as 55195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human testis using 55195-1-AP (same clone as 55195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human testis using 55195-1-AP (same clone as 55195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human skin using 55195-1-AP (same clone as 55195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using CPSF7 Polyclonal antibody (55195-1-AP)

    IHC staining of human skin using 55195-1-AP (same clone as 55195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CPSF7 Polyclonal antibody (55195-1-AP)

    IF Staining of A431 using 55195-1-AP (same clone as 55195-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (55195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55195-1-PBS in a different storage buffer formulation.

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CPSF7 Polyclonal antibody, PBS Only

CPSF7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55195-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55195-1-PBS targets CPSF7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pre-mRNA cleavage factor I, 59 kDa subunit
Calculated molecular weight 56 kDa
Observed molecular weight 52 kDa, 56 kDa
GenBank accession numberNM_001136040
Gene Symbol CPSF7
Gene ID (NCBI) 79869
RRIDAB_10858797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N684
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.