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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPSF7 Polyclonal antibody

CPSF7 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55195-1-AP

Synonyms

CPSF 59 kDa subunit, CPSF7, FLJ12529

Validation Data Gallery

Applications:
  • WB analysis using 55195-1-AP

    WB analysis using 55195-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 55195-1-AP

    WB analysis of HEK-293 using 55195-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 55195-1-AP

    WB analysis of mouse brain using 55195-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 55195-1-AP

    WB analysis of mouse brain using 55195-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 55195-1-AP

    WB analysis of HeLa using 55195-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 55195-1-AP

    IHC staining of human gliomas using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPSF7 antibody included
  • IHC staining of human gliomas using 55195-1-AP

    IHC staining of human gliomas using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPSF7 antibody included
  • IHC staining of human testis using 55195-1-AP

    IHC staining of human testis using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CPSF7 antibody included
  • IHC staining of human testis using 55195-1-AP

    IHC staining of human testis using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CPSF7 antibody included
  • IHC staining of human skin using 55195-1-AP

    IHC staining of human skin using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CPSF7 antibody included
  • IHC staining of human skin using 55195-1-AP

    IHC staining of human skin using 55195-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CPSF7 antibody included
  • IF Staining of A431 using 55195-1-AP

    IF Staining of A431 using 55195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (55195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CPSF7 Ready-To-Use IHC Kit. CPSF7 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55195-1-AP targets CPSF7 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pre-mRNA cleavage factor I, 59 kDa subunit
Calculated molecular weight 56 kDa
Observed molecular weight 52 kDa, 56 kDa
GenBank accession numberNM_001136040
Gene symbol FLJ12529
Gene ID (NCBI) 79869
RRIDAB_10858797
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

Protocols

Product Specific Protocols
WB protocol for CPSF7 antibody 55195-1-APDownload protocol
IHC protocol for CPSF7 antibody 55195-1-APDownload protocol
IF protocol for CPSF7 antibody 55195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Rep

CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation

Authors - Susu Liu
View Article
humanCoIP

J Mol Cell Biol

U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors

Authors - Zhijie Hu
View Article