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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G2B9

Cat no : CL594-66351

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

Validation Data Gallery

Applications:
  • IF Staining of mouse lung using CL594-66351

    IF Staining of mouse lung using CL594-66351

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using CoraLite®594 COX2/ Cyclooxygenase 2/ PTGS2 antibody (CL594-66351, Clone: 3G2B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse lung using CL594-66351

    IF Staining of mouse lung using CL594-66351

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using CoraLite®594 COX2/ Cyclooxygenase 2/ PTGS2 antibody (CL594-66351, Clone: 3G2B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inmouse lung tissue
For other applications, we recommend the unconjugated version of this antibody, 66351-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66351 targets COX2/ Cyclooxygenase 2/ PTGS2 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721 相同性解析による交差性が予測される生物種
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC013734
Gene symbol PTGS2
Gene ID (NCBI) 5743
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).

Protocols

Product Specific Protocols
IF protocol for CL594 COX2/ Cyclooxygenase 2/ PTGS2 antibody CL594-66351Download protocol
Standard Protocols
Click here to view our Standard Protocols