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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HEK-293 using 67218-1-Ig (same clone as 67218-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of Jurkat using 67218-1-Ig (same clone as 67218-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using COIL Monoclonal antibody (67218-1-Ig)

    FC experiment of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human COIL (67218-1-Ig, Clone:2G12B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

COIL Monoclonal antibody, PBS Only

COIL Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67218-1-PBS
Clone No.2G12B8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Coilin, 2G12B8, CLN80, p80 coilin, p80-coilin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67218-1-PBS targets COIL in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17171

Product name: Recombinant human COIL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 212-576 aa of BC010385

Sequence: ANQRCSSPKGSARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELSKEEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGFLKTVGLFAGRGRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREENLFSWKGAKGRGMRGRGRGRGHPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPKKDYSLLPLLAAAPQVGEKIAFKLLELTSSYSPDVSDYKEGRILSHNPETQQVDIEILSSLPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDPRLIIESPSNTSSTEPA

相同性解析による交差性が予測される生物種
Full Name coilin
Calculated molecular weight 576 aa, 63 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
RRIDAB_2882509
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP38432
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.