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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HeLa using 67218-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HEK-293 using 67218-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HepG2 using 67218-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of Jurkat using 67218-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    IF Staining of HepG2 using 67218-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    IF Staining of HepG2 using 67218-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using COIL Monoclonal antibody (67218-1-Ig)

    FC experiment of HeLa using 67218-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human COIL (67218-1-Ig, Clone:2G12B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

COIL Monoclonal antibody

COIL Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67218-1-Ig
Clone No.2G12B8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Coilin, 2G12B8, CLN80, p80 coilin, p80-coilin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67218-1-Ig targets COIL in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17171

Product name: Recombinant human COIL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 212-576 aa of BC010385

Sequence: ANQRCSSPKGSARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELSKEEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGFLKTVGLFAGRGRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREENLFSWKGAKGRGMRGRGRGRGHPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPKKDYSLLPLLAAAPQVGEKIAFKLLELTSSYSPDVSDYKEGRILSHNPETQQVDIEILSSLPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDPRLIIESPSNTSSTEPA

相同性解析による交差性が予測される生物種
Full Name coilin
Calculated molecular weight 576 aa, 63 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
RRIDAB_2882509
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP38432
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COIL antibody 67218-1-IgDownload protocol
IF protocol for COIL antibody 67218-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols