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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COA4 Recombinant antibody (83326-1-RR)

    WB analysis using 83326-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83326-1-RR (COA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using COA4 Recombinant antibody (83326-1-RR)

    IHC staining of human ovarian cancer using 83326-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83326-1-RR (COA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using COA4 Recombinant antibody (83326-1-RR)

    IHC staining of human ovarian cancer using 83326-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83326-1-RR (COA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COA4 Recombinant antibody (83326-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83326-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83326-1-RR (COA4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83326-1-RR

    Affinity and Kinetic Characterization of 83326-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83326-1-RR against Human COA4 were performed. The affinity constant is 0.307 nM.

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COA4 Recombinant antibody

COA4 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83326-1-RR
Clone No.230070G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CHCHD8, E2IG2, E2 induced gene 2 protein, 230070G9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, K-562 cells, HEK-293 cells, PC-3 cells, LNCaP cells, MKN-45 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83326-1-RR targets COA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COA4 fusion protein Ag33966 相同性解析による交差性が予測される生物種
Full Name Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
Calculated molecular weight10 kDa
Observed molecular weight10 kDa
GenBank accession numberNM_016565.2
Gene Symbol COA4
Gene ID (NCBI) 51287
RRIDAB_3670991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NYJ1-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COA4 antibody 83326-1-RRDownload protocol
IHC protocol for COA4 antibody 83326-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols